Labeling and Detection
Labeling and Detection
Labelling biomolecules with fluorophores allows researchers to detect specific components biological systems. Fluorescent detection provides sensitivity in applications ranging from micrsocsopy and imaging, to flow cytometry, to Western blots. Fluorescent labelling is an efficient method for bioanalytical purposes. Fluorophores can easily be attached to many peptides and proteins through conjugation of free thiols or amine side chains. For nucleic acids, introducing modified nucleotides with amine, thiol, or other groups allows fluorescent probes to be covalently attached to DNA and RNA strands. Certain fluorophores can also intercalate into DNA strands allowing for easy detection. Recently, click chemistry has emerged as a convenient method for labelling biomolecules. It involves the reaction of a pair of reagents that are inert to naturally occurring functional groups. Alfa Aesar can provide a variety of fluorophores for labelling biological molecules for fluorescent detection.
| Click Chemistry Reagents | Microspheres | Nanoparticles | Protein and DNA Labeling |